Publications
Oxidative folding and structural analyses of a kunitz-related inhibitor and its disulfide intermediates: functional implications. Bronsoms S, Pantoja-Uceda D, Gabrijelcic-Geiger D, Sanglas L, Aviles FX, Santoro J, Sommerhoff CP, Arolas JL. J Mol Biol. 2011 Dec 2;414(3):427-41. Epub 2011 Oct 18.
Cloning, sequencing, and identification using proteomic tools of a protease from Bromelia hieronymi Mez. Bruno MA, Trejo SA, Avilés FX, Caffini NO, López LM. Appl Biochem Biotechnol. 2011 Sep;165(2):583-93. Epub 2011 May 17.
Biochemical characterization, cDNA cloning, and molecular modeling of araujiain aII, a papain-like cysteine protease from Araujia angustifolia latex. Obregón WD, Lufrano D, Liggieri CS, Trejo SA, Vairo-Cavalli SE, Avilés FX, Priolo NS. Planta. 2011 Aug;234(2):293-304. Epub 2011 Mar 20.
Purification and Characterization of a Cysteine Endopeptidase from Vasconcellea quercifolia A. St.-Hil. Latex Displaying High Substrate Specificity. Torres MJ, Trejo SA, Martin MI, Natalucci CL, Avilés FX, López LM. J Agric Food Chem. 2010 Sep 28. [Epub ahead of print]
Complementary positional proteomics for screening substrates of endo- and exoproteases. Van Damme P, Staes A, Bronsoms S, Helsens K, Colaert N, Timmerman E, Aviles FX, Vandekerckhove J, Gevaert K. Nat Methods. 2010 Jul;7(7):512-5. Epub 2010 Jun 6.
Biochemical and PMF MALDI-TOF analyses of two novel papain-like plant proteinases. Obregón WD, Liggieri CS, Morcelle SR, Trejo SA, Avilés FX, Priolo NS. Protein Pept Lett. 2009;16(11):1323-33.
A novel metallocarboxypeptidase-like enzyme from the marine annelid Sabellastarte magnifica--a step into the invertebrate world of proteases. Alonso-del-Rivero M, Trejo SA, Rodríguez de la Vega M, González Y, Bronsoms S, Canals F, Delfín J, Diaz J, Aviles FX, Chávez MA. FEBS J. 2009 Sep;276(17):4875-90. Epub 2009 Aug 4.
Characterization of papain-like isoenzymes from latex of Asclepias curassavica by molecular biology validated by proteomic approach. Obregón WD, Liggieri CS, Trejo SA, Avilés FX, Vairo-Cavalli SE, Priolo NS. Biochimie. 2009 Nov-Dec;91(11-12):1457-64. Epub 2009 Aug 11.
Designing out disulfide bonds of leech carboxypeptidase inhibitor: implications for its folding, stability and function. Arolas JL, Castillo V, Bronsoms S, Aviles FX, Ventura S. J Mol Biol. 2009 Sep 18;392(2):529-46. Epub 2009 Jun 25.
Insights into the two-domain architecture of the metallocarboxypeptidase inhibitor from the Ascaris parasite inferred from the mechanism of its oxidative folding. Arolas JL, Sanglas L, Lorenzo J, Bronsoms S, Aviles FX. Biochemistry. 2009 Sep 1;48(34):8225-32.
Sequencing and characterization of asclepain f: the first cysteine peptidase cDNA cloned and expressed from Asclepias fruticosa latex. Trejo SA, López LM, Caffini NO, Natalucci CL, Canals F, Avilés FX. Planta. 2009 Jul;230(2):319-28. Epub 2009 May 20.
Heterogeneity of S-layer proteins from aggregating and non-aggregating Lactobacillus kefir strains. Mobili P, Serradell Mde L, Trejo SA, Avilés Puigvert FX, Abraham AG, De Antoni GL. Antonie Van Leeuwenhoek. 2009 May;95(4):363-72. Epub 2009 Mar 22.
Purification and characterization of hieronymain III. Comparison with other proteases previously isolated from Bromelia hieronymi Mez. Bruno MA, Trejo SA, Caffini NO, López LM. Protein J. 2008 Dec;27(7-8):426-33.
Purification and characterization of four new cysteine endopeptidases from fruits of Bromelia pinguin L. grown in Cuba. Payrol JA, Obregón WD, Trejo SA, Caffini NO. Protein J. 2008 Feb;27(2):88-96.
Oxidative folding of leech-derived tryptase inhibitor via native disulfide-bonded intermediates. Arolas JL, Bronsoms S, Aviles FX, Ventura S, Sommerhoff CP. Antioxid Redox Signal. 2008 Jan;10(1):77-85.
Detection of non-covalent protein interactions by 'intensity fading' MALDI-TOF mass spectrometry: applications to proteases and protease inhibitors. Yanes O, Villanueva J, Querol E, Aviles FX. Nat Protoc. 2007;2(1):119-30.
Proteomic profiling of a snake venom using high mass detection MALDI-TOF mass spectrometry. Yanes O, Avilés FX, Wenzel R, Nazabal A, Zenobi R, Calvete JJ. J Am Soc Mass Spectrom. 2007 Apr;18(4):600-6. Epub 2007 Jan 3.
Exploring the "intensity fading" phenomenon in the study of noncovalent interactions by MALDI-TOF mass spectrometry. Yanes O, Aviles FX, Roepstorff P, Jørgensen TJ. J Am Soc Mass Spectrom. 2007 Feb;18(2):359-67. Epub 2006 Nov 9.
Detection of noncovalent complexes in biological samples by intensity fading and high-mass detection MALDI-TOF mass spectrometry. Yanes O, Nazabal A, Wenzel R, Zenobi R, Aviles FX. J Proteome Res. 2006 Oct;5(10):2711-9.
Patents
1.Inventors: Villanueva J., Canals F., Querol E., Avilés F. X., & Serrano L.
"Screening method. Detection and screening of protein-ligand interactions by Maldi-Tof mass spectroscopy".
GB-0031273.6.
2.Inventors: Villanueva J., Yanes O., Serrano L., Avilés F. X. & Querol E
"Procedimiento para la deteccion de la formación de complejos no covalentes biomolécula-ligando"
ES-200200759.
