{"id":5024,"date":"2023-03-10T10:03:11","date_gmt":"2023-03-10T09:03:11","guid":{"rendered":"https:\/\/ibb.uab.cat\/?p=5024"},"modified":"2023-03-10T10:04:31","modified_gmt":"2023-03-10T09:04:31","slug":"nanobiotechnology-protein-structure-biofabrication-of-self-assembling-covalent-protein-nanoparticles-through-histidine-templated-cysteine-coupling","status":"publish","type":"post","link":"https:\/\/ibb.uab.cat\/index.php\/2023\/03\/10\/nanobiotechnology-protein-structure-biofabrication-of-self-assembling-covalent-protein-nanoparticles-through-histidine-templated-cysteine-coupling\/","title":{"rendered":"Nanobiotechnology &#038; Protein Structure: &#8220;Biofabrication of Self-Assembling Covalent Protein Nanoparticles through Histidine-Templated Cysteine Coupling&#8221;"},"content":{"rendered":"\n<p><a href=\"https:\/\/doi.org\/10.1021\/acssuschemeng.2c06635\">https:\/\/doi.org\/10.1021\/acssuschemeng.2c06635<\/a><\/p>\n\n\n\n<p>H\u00e8ctor L\u00f3pez-Laguna,\u00a0Ariana Rueda,\u00a0Carlos Mart\u00ednez-Torr\u00f3,\u00a0Luc\u00eda S\u00e1nchez-Alba,\u00a0Jos\u00e9 Vicente Carratal\u00e1,\u00a0Jan Atienza-Garriga,\u00a0Eloi Parlad\u00e9,\u00a0Julieta M. S\u00e1nchez,\u00a0Naroa Serna,\u00a0Eric Volt\u00e0-Dur\u00e1n,\u00a0Neus Ferrer-Miralles,\u00a0David Reverter,\u00a0Ramon Mangues,\u00a0Antonio Villaverde<strong>*<\/strong>,\u00a0Esther V\u00e1zquez<strong>*<\/strong>,\u00a0and\u00a0Ugutz Unzueta<strong>*<\/strong><\/p>\n\n\n\n<h2 id=\"Abstract\">Abstract<\/h2>\n\n\n\n<div class=\"wp-block-image is-style-default\"><figure class=\"alignright\"><img src=\"https:\/\/pubs.acs.org\/cms\/10.1021\/acssuschemeng.2c06635\/asset\/images\/medium\/sc2c06635_0006.gif\" alt=\"Abstract Image\"\/><\/figure><\/div>\n\n\n\n<p>Nanoscale protein materials show increasing applications in biotechnology and biomedicine, addressing catalysis, drug delivery, or tissue engineering. Although protein oligomerization is reachable through several engineering approaches, including the use of divalent cations for histidine-rich stretches, the effectiveness of cation-His binding is influenced by protein conformation, media composition, and chelating agents. Thus, looking for powerful, green, cross-linker-free, and transversal oligomerization platforms, we have built a histidine-templated cysteine-coupling concept. On this basis, we have engineered a Cys-containing, H6-derived His\u2013Cys hybrid tag that enables the spontaneous and efficient self-assembling of tagged proteins into monodisperse nanoparticles through a highly ordered covalent binding process. Although the generated nanostructures are supported by disulfide bridge formation and exclusively reversed by reducing agents but not by chelating agents, the presence of cysteine residues does not disrupt the metal-binding abilities of histidine residues within the tag. This fact allows one to combine the one-step IMAC-based protein purification and, also, the Zn<sup>2+<\/sup>-induced formation of higher-order microparticulate materials as nanoparticle-releasing protein-only depots. The dual mode of cross-molecular interactivity shown by the hybrid tag and the structural robustness and stability of the resulting nanoparticles offer wide applicability of the green biofabrication concept proposed here for the further development of clinically usable protein materials.<\/p>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>https:\/\/doi.org\/10.1021\/acssuschemeng.2c06635 H\u00e8ctor L\u00f3pez-Laguna,\u00a0Ariana Rueda,\u00a0Carlos Mart\u00ednez-Torr\u00f3,\u00a0Luc\u00eda S\u00e1nchez-Alba,\u00a0Jos\u00e9 Vicente Carratal\u00e1,\u00a0Jan Atienza-Garriga,\u00a0Eloi Parlad\u00e9,\u00a0Julieta M. S\u00e1nchez,\u00a0Naroa Serna,\u00a0Eric Volt\u00e0-Dur\u00e1n,\u00a0Neus Ferrer-Miralles,\u00a0David Reverter,\u00a0Ramon Mangues,\u00a0Antonio Villaverde*,\u00a0Esther V\u00e1zquez*,\u00a0and\u00a0Ugutz Unzueta* Abstract Nanoscale protein materials show increasing applications in biotechnology and biomedicine, addressing catalysis, drug delivery, or tissue engineering. Although protein oligomerization is reachable through several engineering approaches, including the use of divalent cations for histidine-rich [&hellip;]<\/p>\n","protected":false},"author":65,"featured_media":5027,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[4],"tags":[],"_links":{"self":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/5024"}],"collection":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/users\/65"}],"replies":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/comments?post=5024"}],"version-history":[{"count":1,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/5024\/revisions"}],"predecessor-version":[{"id":5025,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/5024\/revisions\/5025"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media\/5027"}],"wp:attachment":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media?parent=5024"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/categories?post=5024"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/tags?post=5024"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}