{"id":4565,"date":"2022-04-06T14:16:31","date_gmt":"2022-04-06T13:16:31","guid":{"rendered":"https:\/\/ibb.uab.cat\/?p=4565"},"modified":"2022-04-06T14:19:50","modified_gmt":"2022-04-06T13:19:50","slug":"nanobiotechnology-all-in-one-biofabrication-and-loading-of-recombinant-vaults-in-human-cells","status":"publish","type":"post","link":"https:\/\/ibb.uab.cat\/index.php\/2022\/04\/06\/nanobiotechnology-all-in-one-biofabrication-and-loading-of-recombinant-vaults-in-human-cells\/","title":{"rendered":"Nanobiotechnology: \u201cAll-in-one biofabrication and loading of recombinant vaults in human cells&#8221;"},"content":{"rendered":"\n<p><a href=\"https:\/\/iopscience.iop.org\/journal\/1758-5090\">Biofabrication<\/a>, <a href=\"https:\/\/iopscience.iop.org\/volume\/1758-5090\/14\">Volume 14<\/a>, <a href=\"https:\/\/iopscience.iop.org\/issue\/1758-5090\/14\/2\">Number 2<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/iopscience.iop.org\/journal\/1758-5090\">https:\/\/iopscience.iop.org\/journal\/1758-5090<\/a><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"alignright size-large is-resized\"><img loading=\"lazy\" src=\"https:\/\/ibb.uab.cat\/wp-content\/uploads\/journal_cover.gif\" alt=\"\" class=\"wp-image-4566\" width=\"206\" height=\"292\"\/><\/figure><\/div>\n\n\n\n<h2 id=\"artAbst\">Abstract<\/h2>\n\n\n\n<p>One of the most promising approaches in the drug delivery field is the use of naturally occurring self-assembling protein nanoparticles, such as virus-like particles, bacterial microcompartments or vault ribonucleoprotein particles as drug delivery systems (DDSs). Among them, eukaryotic vaults show a promising future due to their structural features, <em>in vitro<\/em> stability and non-immunogenicity. Recombinant vaults are routinely produced in insect cells and purified through several ultracentrifugations, both tedious and time-consuming processes. As an alternative, this work proposes a new approach and protocols for the production of recombinant vaults in human cells by transient gene expression of a His-tagged version of the major vault protein (MVP-H6), the development of new affinity-based purification processes for such recombinant vaults, and the all-in-one biofabrication and encapsulation of a cargo recombinant protein within such vaults by their co-expression in human cells. Protocols proposed here allow the easy and straightforward biofabrication and purification of engineered vaults loaded with virtually any INT-tagged cargo protein, in very short times, paving the way to faster and easier engineering and production of better and more efficient DDS.<\/p>\n\n\n\n<p>Fernando Mart\u00edn<sup>7,1<\/sup>, Aida Carre\u00f1o<sup>8,1<\/sup>, Rosa Mendoza<sup>1,2<\/sup>, Pablo Caruana<sup>3<\/sup>, Francisco Rodriguez<sup>3<\/sup>, Marlon Bravo<sup>4,5<\/sup>, Antoni Benito<sup>4,5<\/sup>, Neus Ferrer-Miralles<sup>1,2,6<\/sup>, M Virtudes C\u00e9spedes<sup>9,3<\/sup> and Jos\u00e9 Luis Corchero<sup>9,1,2,6<\/sup><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Biofabrication, Volume 14, Number 2 https:\/\/iopscience.iop.org\/journal\/1758-5090 Abstract One of the most promising approaches in the drug delivery field is the use of naturally occurring self-assembling protein nanoparticles, such as virus-like particles, bacterial microcompartments or vault ribonucleoprotein particles as drug delivery systems (DDSs). Among them, eukaryotic vaults show a promising future due to their structural features, [&hellip;]<\/p>\n","protected":false},"author":65,"featured_media":4566,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[4],"tags":[],"_links":{"self":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/4565"}],"collection":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/users\/65"}],"replies":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/comments?post=4565"}],"version-history":[{"count":5,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/4565\/revisions"}],"predecessor-version":[{"id":4573,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/4565\/revisions\/4573"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media\/4566"}],"wp:attachment":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media?parent=4565"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/categories?post=4565"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/tags?post=4565"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}