{"id":4356,"date":"2021-09-30T17:33:27","date_gmt":"2021-09-30T16:33:27","guid":{"rendered":"https:\/\/ibb.uab.cat\/?p=4356"},"modified":"2021-09-30T17:33:28","modified_gmt":"2021-09-30T16:33:28","slug":"molecular-biology-functional-characterization-of-the-cell-division-gene-cluster-of-the-wall-less-bacterium-mycoplasma-genitalium","status":"publish","type":"post","link":"https:\/\/ibb.uab.cat\/index.php\/2021\/09\/30\/molecular-biology-functional-characterization-of-the-cell-division-gene-cluster-of-the-wall-less-bacterium-mycoplasma-genitalium\/","title":{"rendered":"Molecular Biology: &#8220;Functional Characterization of the Cell Division Gene Cluster of the Wall-less Bacterium Mycoplasma genitalium&#8221;"},"content":{"rendered":"\n<p>Front. Microbiol., 13 September 2021 |\u00a0<a href=\"https:\/\/doi.org\/10.3389\/fmicb.2021.695572\">https:\/\/doi.org\/10.3389\/fmicb.2021.695572<\/a><\/p>\n\n\n\n<p><\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"alignleft size-large is-resized\"><img loading=\"lazy\" src=\"https:\/\/ibb.uab.cat\/wp-content\/uploads\/fmicb-12-695572-g007-1024x876.jpg\" alt=\"\" class=\"wp-image-4358\" width=\"490\" height=\"418\" srcset=\"https:\/\/ibb.uab.cat\/wp-content\/uploads\/fmicb-12-695572-g007-1024x876.jpg 1024w, https:\/\/ibb.uab.cat\/wp-content\/uploads\/fmicb-12-695572-g007-300x257.jpg 300w, https:\/\/ibb.uab.cat\/wp-content\/uploads\/fmicb-12-695572-g007-768x657.jpg 768w, https:\/\/ibb.uab.cat\/wp-content\/uploads\/fmicb-12-695572-g007-1536x1313.jpg 1536w, https:\/\/ibb.uab.cat\/wp-content\/uploads\/fmicb-12-695572-g007-304x260.jpg 304w, https:\/\/ibb.uab.cat\/wp-content\/uploads\/fmicb-12-695572-g007.jpg 1559w\" sizes=\"(max-width: 490px) 100vw, 490px\" \/><\/figure><\/div>\n\n\n\n<p>It is well-established that FtsZ drives peptidoglycan synthesis at the division site in walled bacteria. However, the function and conservation of FtsZ in wall-less prokaryotes such as mycoplasmas are less clear. In the genome-reduced bacterium\u00a0<em>Mycoplasma genitalium<\/em>, the cell division gene cluster is limited to four genes:\u00a0<em>mraZ<\/em>,\u00a0<em>mraW<\/em>, MG_223, and\u00a0<em>ftsZ<\/em>. In a previous study, we demonstrated that\u00a0<em>ftsZ<\/em>\u00a0was dispensable for growth of\u00a0<em>M. genitalium<\/em>\u00a0under laboratory culture conditions. Herein, we show that the entire cell division gene cluster of\u00a0<em>M. genitalium<\/em>\u00a0is non-essential for growth\u00a0<em>in vitro<\/em>. Our analyses indicate that loss of the\u00a0<em>mraZ<\/em>\u00a0gene alone is more detrimental for growth of\u00a0<em>M. genitalium<\/em>\u00a0than deletion of\u00a0<em>ftsZ<\/em>\u00a0or the entire cell division gene cluster. Transcriptional analysis revealed a marked upregulation of\u00a0<em>ftsZ<\/em>\u00a0in the\u00a0<em>mraZ<\/em>\u00a0mutant. Stable isotope labeling by amino acids in cell culture (SILAC)-based proteomics confirmed the overexpression of FtsZ in MraZ-deprived cells. Of note, we found that\u00a0<em>ftsZ<\/em>\u00a0expression was upregulated in non-adherent cells of\u00a0<em>M. genitalium<\/em>, which arise spontaneously at relatively high rates. Single cell analysis using fluorescent markers showed that FtsZ localization varied throughout the cell cycle of\u00a0<em>M. genitalium<\/em>\u00a0in a coordinated manner with the chromosome and the terminal organelle (TMO). In addition, our results indicate a possible role for the RNA methyltransferase MraW in the regulation of FtsZ expression at the post-transcriptional level. Altogether, this study provides an extensive characterization of the cell division gene cluster of\u00a0<em>M. genitalium<\/em>\u00a0and demonstrates the existence of regulatory elements controlling FtsZ expression at the temporal and spatial level in mycoplasmas.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Front. Microbiol., 13 September 2021 |\u00a0https:\/\/doi.org\/10.3389\/fmicb.2021.695572 It is well-established that FtsZ drives peptidoglycan synthesis at the division site in walled bacteria. However, the function and conservation of FtsZ in wall-less prokaryotes such as mycoplasmas are less clear. In the genome-reduced bacterium\u00a0Mycoplasma genitalium, the cell division gene cluster is limited to four genes:\u00a0mraZ,\u00a0mraW, MG_223, and\u00a0ftsZ. In [&hellip;]<\/p>\n","protected":false},"author":65,"featured_media":4358,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[4],"tags":[],"_links":{"self":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/4356"}],"collection":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/users\/65"}],"replies":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/comments?post=4356"}],"version-history":[{"count":1,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/4356\/revisions"}],"predecessor-version":[{"id":4359,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/4356\/revisions\/4359"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media\/4358"}],"wp:attachment":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media?parent=4356"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/categories?post=4356"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/tags?post=4356"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}