{"id":4288,"date":"2021-09-13T08:59:50","date_gmt":"2021-09-13T07:59:50","guid":{"rendered":"https:\/\/ibb.uab.cat\/?p=4288"},"modified":"2021-09-13T09:02:11","modified_gmt":"2021-09-13T08:02:11","slug":"protein-folding-and-conformational-diseases-biparatopic-protein-nanoparticles-for-the-precision-therapy-of-cxcr4-cancers","status":"publish","type":"post","link":"https:\/\/ibb.uab.cat\/index.php\/2021\/09\/13\/protein-folding-and-conformational-diseases-biparatopic-protein-nanoparticles-for-the-precision-therapy-of-cxcr4-cancers\/","title":{"rendered":"Nanobiotechnology: &#8220;Biparatopic Protein Nanoparticles for the Precision Therapy of CXCR4+ Cancers&#8221;"},"content":{"rendered":"\n<p><em>Cancers<\/em>\u00a0<strong>2021<\/strong>,\u00a0<em>13<\/em>(12), 2929<\/p>\n\n\n\n<p>\u00a0<a href=\"https:\/\/doi.org\/10.3390\/cancers13122929\">https:\/\/doi.org\/10.3390\/cancers13122929<\/a><\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"alignleft size-large is-resized\"><img loading=\"lazy\" src=\"https:\/\/ibb.uab.cat\/wp-content\/uploads\/cancers-13-02929-ag-1024x931.png\" alt=\"\" class=\"wp-image-4289\" width=\"360\" height=\"327\" srcset=\"https:\/\/ibb.uab.cat\/wp-content\/uploads\/cancers-13-02929-ag-1024x931.png 1024w, https:\/\/ibb.uab.cat\/wp-content\/uploads\/cancers-13-02929-ag-300x273.png 300w, https:\/\/ibb.uab.cat\/wp-content\/uploads\/cancers-13-02929-ag-768x698.png 768w, https:\/\/ibb.uab.cat\/wp-content\/uploads\/cancers-13-02929-ag-286x260.png 286w, https:\/\/ibb.uab.cat\/wp-content\/uploads\/cancers-13-02929-ag.png 1210w\" sizes=\"(max-width: 360px) 100vw, 360px\" \/><\/figure><\/div>\n\n\n\n<h2>Abstract<\/h2>\n\n\n\n<p>The accumulated molecular knowledge about human cancer enables the identification of multiple cell surface markers as highly specific therapeutic targets. A proper tumor targeting could significantly avoid drug exposure of healthy cells, minimizing side effects, but it is also expected to increase the therapeutic index. Specifically, colorectal cancer has a particularly poor prognosis in late stages, being drug targeting an appropriate strategy to substantially improve the therapeutic efficacy. In this study, we have explored the potential of the human albumin-derived peptide, EPI-X4, as a suitable ligand to target colorectal cancer via the cell surface protein CXCR4, a chemokine receptor overexpressed in cancer stem cells. To explore the potential use of this ligand, self-assembling protein nanoparticles have been generated displaying an engineered EPI-X4 version, which conferred a modest CXCR4 targeting and fast and high level of cell apoptosis in tumor CXCR4<sup>+<\/sup>&nbsp;cells, in vitro and in vivo. In addition, when EPI-X4-based building blocks are combined with biologically inert polypeptides containing the CXCR4 ligand T22, the resulting biparatopic nanoparticles show a dramatically improved biodistribution in mouse models of CXCR4<sup>+<\/sup>&nbsp;human cancer, faster cell internalization and enhanced target cell death when compared to the version based on a single ligand. The generation of biparatopic materials opens exciting possibilities in oncotherapies based on high precision drug delivery based on the receptor CXCR4.&nbsp;<a href=\"https:\/\/www.mdpi.com\/2072-6694\/13\/12\/2929\/htm\">View Full-Text<\/a><em>Keywords:&nbsp;<\/em><a href=\"https:\/\/www.mdpi.com\/search?q=tumor%20homing\">tumor homing<\/a>;&nbsp;<a href=\"https:\/\/www.mdpi.com\/search?q=EPI-X4\">EPI-X4<\/a>;&nbsp;<a href=\"https:\/\/www.mdpi.com\/search?q=CXCR4\">CXCR4<\/a>;&nbsp;<a href=\"https:\/\/www.mdpi.com\/search?q=biparatopic%20nanoparticles\">biparatopic nanoparticles<\/a>;&nbsp;<a href=\"https:\/\/www.mdpi.com\/search?q=tumor%20targeting\">tumor targeting<\/a>;&nbsp;<a href=\"https:\/\/www.mdpi.com\/search?q=drug%20delivery\">drug delivery<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Cancers\u00a02021,\u00a013(12), 2929 \u00a0https:\/\/doi.org\/10.3390\/cancers13122929 Abstract The accumulated molecular knowledge about human cancer enables the identification of multiple cell surface markers as highly specific therapeutic targets. A proper tumor targeting could significantly avoid drug exposure of healthy cells, minimizing side effects, but it is also expected to increase the therapeutic index. Specifically, colorectal cancer has a particularly [&hellip;]<\/p>\n","protected":false},"author":65,"featured_media":4289,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[4],"tags":[],"_links":{"self":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/4288"}],"collection":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/users\/65"}],"replies":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/comments?post=4288"}],"version-history":[{"count":2,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/4288\/revisions"}],"predecessor-version":[{"id":4291,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/4288\/revisions\/4291"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media\/4289"}],"wp:attachment":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media?parent=4288"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/categories?post=4288"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/tags?post=4288"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}