{"id":3930,"date":"2021-03-17T11:31:31","date_gmt":"2021-03-17T10:31:31","guid":{"rendered":"https:\/\/ibb.uab.cat\/?p=3930"},"modified":"2021-04-13T11:32:07","modified_gmt":"2021-04-13T10:32:07","slug":"nanobiotechnology-selecting-subpopulations-of-high-quality-protein-conformers-among-conformational-mixtures-of-recombinant-bovine-mmp-9-solubilized-from-inclusion-bodies","status":"publish","type":"post","link":"https:\/\/ibb.uab.cat\/index.php\/2021\/03\/17\/nanobiotechnology-selecting-subpopulations-of-high-quality-protein-conformers-among-conformational-mixtures-of-recombinant-bovine-mmp-9-solubilized-from-inclusion-bodies\/","title":{"rendered":"Nanobiotechnology: \u201cSelecting Subpopulations of High-Quality Protein Conformers among Conformational Mixtures of Recombinant Bovine MMP-9 Solubilized from Inclusion Bodies&#8221;"},"content":{"rendered":"<div class=\"custom-accordion-for-small-screen-link active\">\n<p><em><img loading=\"lazy\" class=\"size-full wp-image-3791 alignleft\" src=\"https:\/\/ibb.uab.cat\/wp-content\/uploads\/International-Jurnal-of-molecular-sciences_cover-e1607340127155.jpg\" alt=\"\" width=\"400\" height=\"566\" \/>Int. J. Mol. Sci.<\/em>\u202f<b>2021<\/b>,\u202f<em>22<\/em>(6), 3020;\u202f<a href=\"https:\/\/doi.org\/10.3390\/ijms22063020\">https:\/\/doi.org\/10.3390\/ijms22063020<\/a><\/p>\n<h2 style=\"text-align: justify;\">Abstract<\/h2>\n<\/div>\n<div class=\"target-item custom-accordion-for-small-screen-content \">\n<div class=\"art-abstract in-tab hypothesis_container\" style=\"text-align: justify;\">A detailed workflow to analyze the physicochemical characteristics of mammalian matrix metalloproteinase (MMP-9) protein species obtained from protein aggregates (inclusion bodies\u2014IBs) was followed. MMP-9 was recombinantly produced in the prokaryotic microbial cell factories\u202f<span class=\"html-italic\">Clearcoli<\/span>\u202f(an engineered form of\u202f<span class=\"html-italic\">Escherichia coli<\/span>) and\u202f<span class=\"html-italic\">Lactococcus lactis,<\/span>\u202fmainly forming part of IBs and partially recovered under non-denaturing conditions. After the purification by affinity chromatography of solubilized MMP-9, four protein peaks were obtained. However, so far, the different conformational protein species forming part of IBs have not been isolated and characterized. Therefore, with the aim to link the physicochemical characteristics of the isolated peaks with their biological activity, we set up a methodological approach that included dynamic light scattering (DLS), circular dichroism (CD), and spectrofluorometric analysis confirming the separation of subpopulations of conformers with specific characteristics. In protein purification procedures, the detailed analysis of the individual physicochemical properties and the biological activity of protein peaks separated by chromatographic techniques is a reliable source of information to select the best-fitted protein populations.\u202f<a href=\"https:\/\/www.mdpi.com\/1422-0067\/22\/6\/3020\/htm\">View Full-Text<\/a><\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Int. J. Mol. Sci.\u202f2021,\u202f22(6), 3020;\u202fhttps:\/\/doi.org\/10.3390\/ijms22063020 Abstract A detailed workflow to analyze the physicochemical characteristics of mammalian matrix metalloproteinase (MMP-9) protein species obtained from protein aggregates (inclusion bodies\u2014IBs) was followed. MMP-9 was recombinantly produced in the prokaryotic microbial cell factories\u202fClearcoli\u202f(an engineered form of\u202fEscherichia coli) and\u202fLactococcus lactis,\u202fmainly forming part of IBs and partially recovered under non-denaturing conditions. [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[4],"tags":[],"_links":{"self":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/3930"}],"collection":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/comments?post=3930"}],"version-history":[{"count":1,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/3930\/revisions"}],"predecessor-version":[{"id":3931,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/3930\/revisions\/3931"}],"wp:attachment":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media?parent=3930"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/categories?post=3930"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/tags?post=3930"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}