{"id":2765,"date":"2020-02-26T17:47:46","date_gmt":"2020-02-26T16:47:46","guid":{"rendered":"https:\/\/ibb.uab.cat\/?p=2765"},"modified":"2020-02-26T18:03:06","modified_gmt":"2020-02-26T17:03:06","slug":"dra-nerea-roher-and-dr-a-villaverde-nanostructured-recombinant-protein-particles-raise-specific-antibodies-against-the-nodavirus-nnv-coat-protein-in-sole","status":"publish","type":"post","link":"https:\/\/ibb.uab.cat\/index.php\/2020\/02\/26\/dra-nerea-roher-and-dr-a-villaverde-nanostructured-recombinant-protein-particles-raise-specific-antibodies-against-the-nodavirus-nnv-coat-protein-in-sole\/","title":{"rendered":"Dra. Nerea Roher and Dr. A Villaverde: Nanostructured recombinant protein particles raise specific antibodies against the nodavirus NNV coat protein in sole"},"content":{"rendered":"

https:\/\/doi.org\/10.1016\/j.fsi.2020.02.029<\/p>\n

\n
\n

Highlights<\/h2>\n
\n

We test a nanoparticle vaccine made of nodavirus RGNNV\/SJNNV coat protein in sole.<\/p>\n

Oral and i.p. delivery routes were compared via antibody and gene expression data.<\/p>\n

Injection without adjuvant triggered a strong systemic specific antibody response.<\/p>\n

Oral intubation evoked mainly a mucosal immune response.<\/p>\n

Nanoparticle VNNV-CNP<\/sup>\u00a0is immunogenic in\u00a0S. senegalensis<\/em>\u00a0juveniles.<\/p>\n<\/div>\n

\"\"<\/p>\n<\/div>\n

\n

Abstract<\/h2>\n
\n

Nervous necrosis virus (NNV) reassortant strains RGNNV\/SJNNV have emerged as a potent threat to the Mediterranean marine aquaculture industry, causing viral encephalopathy and retinopathy (VER) in Senegalese sole (Solea senegalensis<\/em>). In this study, a cheap and practical vaccine strategy using bacterial inclusion bodies made of the coat protein of a virulent reassortant strain of this betanodavirus was devised. The nanostructured recombinant protein nanoparticles, VNNV-CNP<\/sup>, were administered without adjuvant to two groups of juvenile sole, one by intraperitoneal injection and the other by oral intubation. Specific antibodies were raised\u00a0in vivo<\/em>\u00a0against the NNV coat protein via both routes, with a substantial specific antibody expansion in the injected group 30 days post homologous prime boost. Expression levels of five adaptive immune-related genes,\u00a0cd8a, cd4, igm, igt<\/em>\u00a0and\u00a0arg2,<\/em>\u00a0were also quantified in intestine, spleen and head kidney. Results showed\u00a0cd4<\/em>\u00a0and\u00a0igm<\/em>\u00a0were upregulated in the head kidney of injected fish, indicating activation of an adaptive systemic response, while intubated fish exhibited a mucosal response in the intestine. Neither route showed significant differential expression of\u00a0cd8a<\/em>. The specific antibody response elicited\u00a0in vivo<\/em>\u00a0and the lack of any signs of toxicity over the 6-week study period in young fish (n\u202f=\u202f100), evidences the potential of the nanoparticle as a vaccine candidate.<\/p>\n

 <\/p>\n<\/div>\n<\/div>\n<\/div>\n

\n
\n
\u00a0<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

https:\/\/doi.org\/10.1016\/j.fsi.2020.02.029 Highlights We test a nanoparticle vaccine made of nodavirus RGNNV\/SJNNV coat protein in sole. Oral and i.p. delivery routes were compared via antibody and gene expression data. Injection without adjuvant triggered a strong systemic specific antibody response. Oral intubation evoked mainly a mucosal immune response. Nanoparticle VNNV-CNP\u00a0is immunogenic in\u00a0S. senegalensis\u00a0juveniles. Abstract Nervous necrosis virus […]<\/p>\n","protected":false},"author":65,"featured_media":2767,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[4],"tags":[],"_links":{"self":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/2765"}],"collection":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/users\/65"}],"replies":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/comments?post=2765"}],"version-history":[{"count":3,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/2765\/revisions"}],"predecessor-version":[{"id":2770,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/2765\/revisions\/2770"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media\/2767"}],"wp:attachment":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media?parent=2765"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/categories?post=2765"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/tags?post=2765"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}