{"id":2665,"date":"2020-01-14T09:55:27","date_gmt":"2020-01-14T08:55:27","guid":{"rendered":"https:\/\/ibb.uab.cat\/?p=2665"},"modified":"2020-01-14T09:55:27","modified_gmt":"2020-01-14T08:55:27","slug":"dr-salvador-ventura-c-mannosylation-supports-folding-and-enhances-stability-of-thrombospondin-repeats","status":"publish","type":"post","link":"https:\/\/ibb.uab.cat\/index.php\/2020\/01\/14\/dr-salvador-ventura-c-mannosylation-supports-folding-and-enhances-stability-of-thrombospondin-repeats\/","title":{"rendered":"Dr. Salvador Ventura (C-mannosylation supports folding and enhances stability of thrombospondin repeats)"},"content":{"rendered":"<header class=\"article-section__header\">\n<h2 class=\"article-section__header_text\" style=\"text-align: justify;\"><img loading=\"lazy\" class=\"wp-image-2666 alignleft\" src=\"https:\/\/ibb.uab.cat\/wp-content\/uploads\/salva.jpg\" alt=\"\" width=\"542\" height=\"452\" srcset=\"https:\/\/ibb.uab.cat\/wp-content\/uploads\/salva.jpg 1191w, https:\/\/ibb.uab.cat\/wp-content\/uploads\/salva-300x250.jpg 300w, https:\/\/ibb.uab.cat\/wp-content\/uploads\/salva-768x640.jpg 768w, https:\/\/ibb.uab.cat\/wp-content\/uploads\/salva-1024x854.jpg 1024w, https:\/\/ibb.uab.cat\/wp-content\/uploads\/salva-312x260.jpg 312w\" sizes=\"(max-width: 542px) 100vw, 542px\" \/><strong><span style=\"font-size: 12pt;\">https:\/\/elifesciences.org\/articles\/52978<\/span><\/strong><\/h2>\n<h2 class=\"article-section__header_text\" style=\"text-align: justify;\">Abstract<\/h2>\n<\/header>\n<div class=\"article-section__body\">\n<p class=\"paragraph\" style=\"text-align: justify;\">Previous studies demonstrated importance of C-mannosylation for efficient protein secretion. To study its impact on protein folding and stability, we analyzed both C-mannosylated and non-C-mannosylated thrombospondin type 1 repeats (TSRs) of netrin receptor UNC-5. In absence of C-mannosylation, UNC-5 TSRs could only be obtained at low temperature and a significant proportion displayed incorrect intermolecular disulfide bridging, which was hardly observed when C-mannosylated. Glycosylated TSRs exhibited higher resistance to thermal and reductive denaturation processes, and the presence of C-mannoses promoted the oxidative folding of a reduced and denatured TSR in vitro. Molecular dynamics simulations supported the experimental studies and showed that C-mannoses can be involved in intramolecular hydrogen bonding and limit the flexibility of the TSR tryptophan-arginine ladder. We propose that in the endoplasmic reticulum folding process, C-mannoses orient the underlying tryptophan residues and facilitate the formation of the tryptophan-arginine ladder, thereby influencing the positioning of cysteines and disulfide bridging.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>https:\/\/elifesciences.org\/articles\/52978 Abstract Previous studies demonstrated importance of C-mannosylation for efficient protein secretion. To study its impact on protein folding and stability, we analyzed both C-mannosylated and non-C-mannosylated thrombospondin type 1 repeats (TSRs) of netrin receptor UNC-5. In absence of C-mannosylation, UNC-5 TSRs could only be obtained at low temperature and a significant proportion displayed incorrect [&hellip;]<\/p>\n","protected":false},"author":65,"featured_media":2666,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[4],"tags":[],"_links":{"self":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/2665"}],"collection":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/users\/65"}],"replies":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/comments?post=2665"}],"version-history":[{"count":1,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/2665\/revisions"}],"predecessor-version":[{"id":2667,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/2665\/revisions\/2667"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media\/2666"}],"wp:attachment":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media?parent=2665"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/categories?post=2665"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/tags?post=2665"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}