{"id":2641,"date":"2019-12-20T09:07:00","date_gmt":"2019-12-20T08:07:00","guid":{"rendered":"https:\/\/ibb.uab.cat\/?p=2641"},"modified":"2019-12-20T09:08:01","modified_gmt":"2019-12-20T08:08:01","slug":"dr-a-villaverde-engineering-a-nanostructured-nucleolin-binding-peptide-for-intracellular-drug-delivery-in-triple-negative-breast-cancer-stem-cells","status":"publish","type":"post","link":"https:\/\/ibb.uab.cat\/index.php\/2019\/12\/20\/dr-a-villaverde-engineering-a-nanostructured-nucleolin-binding-peptide-for-intracellular-drug-delivery-in-triple-negative-breast-cancer-stem-cells\/","title":{"rendered":"Dr. A. Villaverde: Engineering a Nanostructured Nucleolin-Binding Peptide for Intracellular Drug Delivery in Triple-Negative Breast Cancer Stem Cells"},"content":{"rendered":"<h2 id=\"Abstract\" class=\"article_abstract-title\" style=\"text-align: justify;\"><img loading=\"lazy\" class=\"size-full wp-image-2642 alignleft\" src=\"https:\/\/ibb.uab.cat\/wp-content\/uploads\/villaverdelargecover.jpg\" alt=\"\" width=\"275\" height=\"365\" srcset=\"https:\/\/ibb.uab.cat\/wp-content\/uploads\/villaverdelargecover.jpg 275w, https:\/\/ibb.uab.cat\/wp-content\/uploads\/villaverdelargecover-226x300.jpg 226w, https:\/\/ibb.uab.cat\/wp-content\/uploads\/villaverdelargecover-196x260.jpg 196w\" sizes=\"(max-width: 275px) 100vw, 275px\" \/>Abstract<\/h2>\n<div id=\"abstractBox\" class=\"article_abstract-content hlFld-Abstract\">\n<p class=\"articleBody_abstractText\" style=\"text-align: justify;\">Five peptide ligands of four different cell surface receptors (nucleolin, CXCR1 CMKLR1 and CD44v6) have been evaluated as targeting moieties for triple-negative human breast cancers. Among them, the peptide F3, derived from phage display, promotes the fast and efficient internalization of a genetically fused GFP inside MDA-MB-231 cancer stem cells, in a specific receptor-dependent fashion. The further engineering of this protein into the modular construct F3-RK-GFP-H6 and the subsequent construct F3-RK-PE24-H6, resulted in self-assembling polypeptides that organize as discrete and regular nanoparticles. These materials, of 15-20 nm in size, show enhanced nucleolin-dependent cell penetrability. We show that the F3-RK-PE24-H6, based on the Pseudomonas aeruginosa exotoxin A (PE24) as a core functional domain, is highly cytotoxic over target cells. The combination of F3, the cationic peptide (RK)n, and the toxin domain PE24 in such unusual presentation appears as a promising approach to cell-targeted drug carriers in breast cancers and address selective drug delivery in otherwise difficult-to-treat triple-negative breast cancers.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Abstract Five peptide ligands of four different cell surface receptors (nucleolin, CXCR1 CMKLR1 and CD44v6) have been evaluated as targeting moieties for triple-negative human breast cancers. Among them, the peptide F3, derived from phage display, promotes the fast and efficient internalization of a genetically fused GFP inside MDA-MB-231 cancer stem cells, in a specific receptor-dependent [&hellip;]<\/p>\n","protected":false},"author":65,"featured_media":2642,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[4],"tags":[],"_links":{"self":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/2641"}],"collection":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/users\/65"}],"replies":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/comments?post=2641"}],"version-history":[{"count":1,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/2641\/revisions"}],"predecessor-version":[{"id":2643,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/2641\/revisions\/2643"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media\/2642"}],"wp:attachment":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media?parent=2641"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/categories?post=2641"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/tags?post=2641"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}