{"id":2584,"date":"2019-12-09T10:47:21","date_gmt":"2019-12-09T09:47:21","guid":{"rendered":"https:\/\/ibb.uab.cat\/?p=2584"},"modified":"2019-12-09T10:48:50","modified_gmt":"2019-12-09T09:48:50","slug":"dr-a-villaverde-endosomal-escape-of-protein-nanoparticles-engineered-through-humanized-histidine-rich-peptides","status":"publish","type":"post","link":"https:\/\/ibb.uab.cat\/index.php\/2019\/12\/09\/dr-a-villaverde-endosomal-escape-of-protein-nanoparticles-engineered-through-humanized-histidine-rich-peptides\/","title":{"rendered":"Dr. A. Villaverde: Endosomal escape of protein nanoparticles engineered through humanized histidine-rich peptides"},"content":{"rendered":"<p><img loading=\"lazy\" class=\"size-full wp-image-2585 alignleft\" src=\"https:\/\/ibb.uab.cat\/wp-content\/uploads\/5dcc9fe06b529d6b2a55be5c.jpg\" alt=\"\" width=\"234\" height=\"314\" srcset=\"https:\/\/ibb.uab.cat\/wp-content\/uploads\/5dcc9fe06b529d6b2a55be5c.jpg 234w, https:\/\/ibb.uab.cat\/wp-content\/uploads\/5dcc9fe06b529d6b2a55be5c-224x300.jpg 224w, https:\/\/ibb.uab.cat\/wp-content\/uploads\/5dcc9fe06b529d6b2a55be5c-194x260.jpg 194w\" sizes=\"(max-width: 234px) 100vw, 234px\" \/>http:\/\/engine.scichina.com\/publisher\/scp\/journal\/SCMs\/doi\/10.1007\/s40843-019-1231-y?slug=abstract<\/p>\n<p>&nbsp;<\/p>\n<h3>Abstract<\/h3>\n<p class=\"introp\">Poly-histidine peptides such H6 (HHHHHH) are used in protein biotechnologies as purification tags, protein-assembling agents at the nanoscale and endosomal-escape entities. The pleiotropic properties of such peptides make them appealing to design protein-based smart materials or nanoparticles for imaging or drug delivery to be produced in form of recombinant proteins. However, the clinical applicability of H6-tagged proteins is restricted by the potential immunogenicity of these segments. In this study we have explored several humanized histidine-rich peptides in tumor-targeted modular proteins than bind and internalize cells through the tumoral marker CXCR4. We were particularly interested in exploring how protein purification, self-assembling and endosomal escape perform in proteins containing the variant histidine-rich tags. Among the tested candidates, the peptide H5E (HEHEHEHEH) has resulted promising as a good promoter of endosomal escape of the associated full-length protein upon endosomal internalization. The numerical modelling of cell penetration and endosomal escape of the tested proteins has revealed a negative relationship between the amount of protein internalized into target cells and the efficiency of cytoplasmic release. This fact demonstrates that the His-mediated, proton sponge-based endosomal escape saturates at moderate amounts of internalized protein, a fact that might be critical for the design of protein materials for cytosolic molecular delivery.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>http:\/\/engine.scichina.com\/publisher\/scp\/journal\/SCMs\/doi\/10.1007\/s40843-019-1231-y?slug=abstract &nbsp; Abstract Poly-histidine peptides such H6 (HHHHHH) are used in protein biotechnologies as purification tags, protein-assembling agents at the nanoscale and endosomal-escape entities. The pleiotropic properties of such peptides make them appealing to design protein-based smart materials or nanoparticles for imaging or drug delivery to be produced in form of recombinant proteins. However, the [&hellip;]<\/p>\n","protected":false},"author":65,"featured_media":2585,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[4],"tags":[],"_links":{"self":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/2584"}],"collection":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/users\/65"}],"replies":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/comments?post=2584"}],"version-history":[{"count":1,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/2584\/revisions"}],"predecessor-version":[{"id":2586,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/2584\/revisions\/2586"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media\/2585"}],"wp:attachment":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media?parent=2584"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/categories?post=2584"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/tags?post=2584"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}