{"id":2521,"date":"2019-09-26T09:12:25","date_gmt":"2019-09-26T08:12:25","guid":{"rendered":"https:\/\/ibb.uab.cat\/?p=2521"},"modified":"2019-09-26T09:12:52","modified_gmt":"2019-09-26T08:12:52","slug":"dr-a-villaverder-high-throughput-cell-motility-studies-on-surface-bound-protein-nanoparticles-with-diverse-structural-and-compositional-characteristics","status":"publish","type":"post","link":"https:\/\/ibb.uab.cat\/index.php\/2019\/09\/26\/dr-a-villaverder-high-throughput-cell-motility-studies-on-surface-bound-protein-nanoparticles-with-diverse-structural-and-compositional-characteristics\/","title":{"rendered":"Dr. A. Villaverder: &#8220;High-Throughput Cell Motility Studies on Surface-Bound Protein Nanoparticles with Diverse Structural and Compositional Characteristics&#8221;"},"content":{"rendered":"<p>&nbsp;<\/p>\n<p><i>ACS Biomater. Sci. Eng.<\/i>2019<\/p>\n<p>https:\/\/pubs.acs.org\/doi\/abs\/10.1021\/acsbiomaterials.9b01085<\/p>\n<p>&nbsp;<\/p>\n<h2 id=\"Abstract\" class=\"article_abstract-title\">Abstract<\/h2>\n<div id=\"abstractBox\" class=\"article_abstract-content hlFld-Abstract\">\n<div id=\"_i1\" class=\"article_abstract-img\" style=\"text-align: justify;\"><img class=\"alignleft\" src=\"https:\/\/pubs.acs.org\/na101\/home\/literatum\/publisher\/achs\/journals\/content\/abseba\/0\/abseba.ahead-of-print\/acsbiomaterials.9b01085\/20190923\/images\/medium\/ab9b01085_0012.gif\" alt=\"Abstract Image\" \/><\/div>\n<p class=\"articleBody_abstractText\" style=\"text-align: justify;\">Eighty areas with different structural and compositional characteristics made of bacterial inclusion bodies formed by the fibroblast growth factor (FGF-IBs) were simultaneously patterned on a glass surface with an evaporation-assisted method that relies on the coffee-drop effect. The resulting surface patterned with these protein nanoparticles enabled to perform a high-throughput study of the motility of NIH-3T3 fibroblasts under different conditions including the gradient steepness, particle concentrations, and area widths of patterned FGF-IBs, using for the data analysis a methodology that includes \u201cheat maps\u201d. From this analysis, we observed that gradients of concentrations of surface-bound FGF-IBs stimulate the total cell movement but do not affect the total net distances traveled by cells. Moreover, cells tend to move toward an optimal intermediate FGF-IB concentration (i.e., cells seeded on areas with high IB concentrations moved toward areas with lower concentrations and vice versa, reaching the optimal concentration). Additionally, a higher motility was obtained when cells were deposited on narrow and highly concentrated areas with IBs. FGF-IBs can be therefore used to enhance and guide cell migration, confirming that the decoration of surfaces with such IB-like protein nanoparticles is a promising platform for regenerative medicine and tissue engineering.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; ACS Biomater. Sci. Eng.2019 https:\/\/pubs.acs.org\/doi\/abs\/10.1021\/acsbiomaterials.9b01085 &nbsp; Abstract Eighty areas with different structural and compositional characteristics made of bacterial inclusion bodies formed by the fibroblast growth factor (FGF-IBs) were simultaneously patterned on a glass surface with an evaporation-assisted method that relies on the coffee-drop effect. The resulting surface patterned with these protein nanoparticles enabled to [&hellip;]<\/p>\n","protected":false},"author":65,"featured_media":2526,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[4],"tags":[],"_links":{"self":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/2521"}],"collection":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/users\/65"}],"replies":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/comments?post=2521"}],"version-history":[{"count":1,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/2521\/revisions"}],"predecessor-version":[{"id":2525,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/2521\/revisions\/2525"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media\/2526"}],"wp:attachment":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media?parent=2521"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/categories?post=2521"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/tags?post=2521"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}