{"id":2446,"date":"2019-07-10T08:23:38","date_gmt":"2019-07-10T07:23:38","guid":{"rendered":"https:\/\/ibb.uab.cat\/?p=2446"},"modified":"2019-07-10T08:43:47","modified_gmt":"2019-07-10T07:43:47","slug":"dra-aurora-ruiz-herrera-three-dimensional-genomic-structure-and-cohesin-occupancy-correlate-with-transcriptional-activity-during-spermatogenesis","status":"publish","type":"post","link":"https:\/\/ibb.uab.cat\/index.php\/2019\/07\/10\/dra-aurora-ruiz-herrera-three-dimensional-genomic-structure-and-cohesin-occupancy-correlate-with-transcriptional-activity-during-spermatogenesis\/","title":{"rendered":"Dra. Aurora Ruiz-Herrera: Three-Dimensional Genomic Structure and Cohesin Occupancy Correlate with Transcriptional Activity during Spermatogenesis"},"content":{"rendered":"

ARTICLE| VOLUME 28, ISSUE 2, P352-367.E9, JULY 09, 2019<\/p>\n

Covadonga Vara; Andreu Paytuv\u00ed-Gallart ; Yasmina Cuartero ;Paul D. Waters; Marc A. Marti-Renom; Aurora Ruiz-Herrera<\/p>\n

Open Access DOI:\u00a0https:\/\/doi.org\/10.1016\/j.celrep.2019.06.037<\/a><\/p>\n

\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 https:\/\/www.cell.com\/cell-reports\/fulltext\/S2211-1247(19)30803-4<\/p>\n

\"\"Summary<\/span><\/h2>\n
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Mammalian gametogenesis involves dramatic and tightly regulated chromatin remodeling, whose regulatory pathways remain largely unexplored. Here, we generate a comprehensive high-resolution structural and functional atlas of mouse spermatogenesis by combining\u00a0in situ<\/em>\u00a0chromosome conformation capture sequencing (Hi-C), RNA sequencing (RNA-seq), and chromatin immunoprecipitation sequencing (ChIP-seq) of CCCTC-binding factor (CTCF) and meiotic cohesins, coupled with confocal\u00a0<\/div>\n

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and super-resolution microscopy. Spermatogonia presents well-defined compartment patterns and topological domains. However, chromosome occupancy and compartmentalization are highly re-arranged during prophase\u00a0I, with cohesins bound to active promoters in DNA loops out of the chromosomal axes. Compartment patterns re-emerge in round spermatids, where cohesin occupancy correlates with transcriptional activity of key developmental genes. The compact sperm genome contains compartments with actively transcribed genes but no fine-scale topological domains, concomitant with the presence of protamines. Overall, we demonstrate how genome-wide cohesin occupancy and transcriptional activity is associated with three-dimensional (3D) remodeling during spermatogenesis, ultimately reprogramming the genome for the next generation.<\/div>\n<\/div>\n
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ARTICLE| VOLUME 28, ISSUE 2, P352-367.E9, JULY 09, 2019 Covadonga Vara; Andreu Paytuv\u00ed-Gallart ; Yasmina Cuartero ;Paul D. Waters; Marc A. Marti-Renom; Aurora Ruiz-Herrera Open Access DOI:\u00a0https:\/\/doi.org\/10.1016\/j.celrep.2019.06.037 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 […]<\/p>\n","protected":false},"author":65,"featured_media":2449,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[4],"tags":[],"_links":{"self":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/2446"}],"collection":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/users\/65"}],"replies":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/comments?post=2446"}],"version-history":[{"count":1,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/2446\/revisions"}],"predecessor-version":[{"id":2450,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/2446\/revisions\/2450"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media\/2449"}],"wp:attachment":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media?parent=2446"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/categories?post=2446"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/tags?post=2446"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}