{"id":1277,"date":"2017-11-16T08:30:24","date_gmt":"2017-11-16T07:30:24","guid":{"rendered":"http:\/\/ibb.uab.cat\/?p=1277"},"modified":"2017-11-16T08:31:24","modified_gmt":"2017-11-16T07:31:24","slug":"dr-salvador-ventura-a-single-cysteine-posttranslational-oxidation-suffices-to-compromise-globular-proteins-kinetic-stability-and-promote-amyloid-formation","status":"publish","type":"post","link":"https:\/\/ibb.uab.cat\/index.php\/2017\/11\/16\/dr-salvador-ventura-a-single-cysteine-posttranslational-oxidation-suffices-to-compromise-globular-proteins-kinetic-stability-and-promote-amyloid-formation\/","title":{"rendered":"Dr. Salvador Ventura “A single cysteine posttranslational oxidation suffices to compromise globular proteins kinetic stability and promote amyloid formation”"},"content":{"rendered":"

\"\"<\/a><\/span><\/span><\/a><\/span><\/h1>\n

Abstract<\/h2>\n
\n

Oxidatively modified forms of proteins accumulate during aging. Oxidized protein conformers might act as intermediates in the formation of\u00a0amyloids<\/a>\u00a0in age-related disorders. However, it is not known whether this amyloidogenic conversion requires an extensive protein\u00a0oxidative damage<\/a>\u00a0or it can be promoted just by a discrete, localized\u00a0post-translational modification<\/a>\u00a0of certain residues. Here, we demonstrate that the irreversible oxidation of a single free\u00a0Cys<\/a>suffices to severely perturb the folding\u00a0energy landscape<\/a>\u00a0of a stable\u00a0globular protein<\/a>, compromise its kinetic stability, and lead to the formation of amyloids under physiological conditions. Experiments and simulations converge to indicate that this specific oxidation-promoted\u00a0protein aggregation<\/a>\u00a0requires only local unfolding. Indeed, a large scale analysis indicates that many cellular proteins are at risk of undergoing this kind of deleterious transition; explaining how oxidative stress can impact cell proteostasis and subsequently lead to the onset of pathological states.<\/p>\n<\/div>\n

Patrizia<\/span>Marinelli<\/span>a<\/sup><\/span>1<\/sup><\/span><\/span><\/a>Susanna<\/span>Navarro<\/span>a<\/sup><\/span>1<\/sup><\/span><\/span><\/a>Ricardo<\/span>Gra\u00f1a<\/span>a<\/sup><\/span><\/span><\/a>Manuel<\/span>Ba\u00f1o-Polo<\/span>a<\/sup><\/span><\/span><\/a>Maria Rosario<\/span>F\u00e9rnandez<\/span>a<\/sup><\/span><\/span><\/a>Elena<\/span>Papaleo<\/span>b<\/sup><\/span><\/span><\/a>Salvador<\/span>Ventura<\/span>a<\/sup><\/span><\/span><\/a><\/span><\/h2>\n","protected":false},"excerpt":{"rendered":"

Abstract Oxidatively modified forms of proteins accumulate during aging. Oxidized protein conformers might act as intermediates in the formation of\u00a0amyloids\u00a0in age-related disorders. However, it is not known whether this amyloidogenic conversion requires an extensive protein\u00a0oxidative damage\u00a0or it can be promoted just by a discrete, localized\u00a0post-translational modification\u00a0of certain residues. Here, we demonstrate that the irreversible oxidation […]<\/p>\n","protected":false},"author":65,"featured_media":1279,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[4],"tags":[],"_links":{"self":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/1277"}],"collection":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/users\/65"}],"replies":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/comments?post=1277"}],"version-history":[{"count":2,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/1277\/revisions"}],"predecessor-version":[{"id":1281,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/posts\/1277\/revisions\/1281"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media\/1279"}],"wp:attachment":[{"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/media?parent=1277"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/categories?post=1277"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ibb.uab.cat\/index.php\/wp-json\/wp\/v2\/tags?post=1277"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}